If you believe you are ready for one of the following cycles you will have many cycles under your belt, supplemented with numerous anabolic steroids , 5 is a good number and have learned how to not only have successful cycles but ones in-which side-effects are controlled. Further, to be ready for an advanced cycle you will be one who is able to maintain more gains after a cycle than you lose. Some gains will always be lost after a cycle is complete, this is a given but maintaining a majority of your gains is possible. If this is not possible for you then you need to reexamine your use, your diet and perhaps even your training.
Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity.